Motility, Adhesion and c-di-GMP Influence the Endophytic Colonization of Rice by Azoarcus sp. CIB

Proficient crop production is needed to ensure the feeding of a growing global population. The association bacteria with plants plays an important role in health state contributing increase agricultural production. Endophytic are ubiquitous most plant species providing, cases, promotion properties. However, knowledge on genetic determinants involved colonization by endophytic still poorly understood. In this work we have used approach based construction fliM, pilX and eps knockout mutants show that motility mediated functional flagellum pili type IV, adhesion modulated exopolysaccarides required for efficient rice roots endophyte Azoarcus sp. CIB. Moreover, demonstrated expression exogenous diguanylate cyclase or phophodiesterase, which causes either decrease intracellular levels second messenger cyclic di-GMP (c-di-GMP), respectively, leads reduction ability CIB colonize plants. Here present results demonstrating unprecedented universal cyclic-di-GMP bacterium, These studies pave way further strategies modulate interaction endophytes their target hosts.